In search of new brain biomarkers of stress

The aim: of the study was to investigate level ghrelin in various brain structures during a stress response Zebrafish predator, evaluate this indicator as potential biomarker stress, and effect benzodiazepine tranquilizer (phenazepam) on stress-induced changes
 Materials methods: object Zebrafish, or Danio rerio wild type, which subjected by exposure predator Hypsophrys nicaraguensis from cichlid family. In tail tissue, cortisol determined, – total (acylated non-acylated) method enzyme-linked immunosorbent assay. anxiolytic phenazepam (1 mg/L), antagonist [D-Lys3]-GHRP-6 (0.333 mg/l) corticotropin-releasing hormone (CRF; 0.4 mg/L) were used pharmacological agents.
 Results discussion: Exposure just administering CRF, more than doubled tissue. prevented an increase tissue level. Simultaneously, medulla oblongata cerebellum, phylogenetically most ancient structures, rather forebrain (telencephalon) midbrain (corpora bigemia), recorded about 500 pg/g protein. increased nanogram concentrations moderately decreased cerebellum. [D-Lys3]-GHRP-6.
 Conclusion: Increases stressful situations can be seen functional along with levels levels. Both these effects are both tranquilizer. mechanism action is antagonism between GABAergic system system.